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. Author manuscript; available in PMC: 2010 Jul 19.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2006 May;47(5):1938–1946. doi: 10.1167/iovs.05-1129

Table 1.

Antibodies Used in Immunofluorescence and Western Blots

Antigen Category Catalog No. Source Working Dilution or Concentration
Indirect Immunofluorescent staining
 SPRR 1A Rabbit polyclonal ab18580 Abcam, Inc., Cambridge, MA 1: 50
 Filaggrin Mouse monoclonal BT-576 Biomedical Technologies Inc, Stoughton, MA 1: 50
Western blot and immunofluorescent staining
 SPRR 2A/2B Rabbit polyclonal APO-25N-001 Alexis Biochemicals, San Diego, CA 1: 500 (WB)
1: 100 (IF)
 Involucrin Mouse monoclonal MS-126, Ab-1 clone SY5 Lab Vision, Fremont, CA 1: 100 or 2 μg/mL (WB and IF)

WB, Western blot; IF, immunofluorescent staining.