Table 2.
Primers Used in Polymerase Chain Reaction
| Cornified Envelope Precursor | NCBI No. | Primer Sequence | Expected Product Size (bp) |
|---|---|---|---|
| SPRR1A | NM 005987 | F: tggccactggatactgaaca R: cccaaatccatcctcaaatg |
230 |
| SPRR1B | NM 003125 | F: cattctgtctcccccaaaaa R: atgggggtataagggagctg |
172 |
| SPRR2A | NM 005988 | F: tatttggctcacctcgttcc R: ccaggacttcctttgctcag |
187 |
| SPRR2B | NM 006945 | F: taattggctcacctcgttcc R: gagctgctgctctttctgct |
229 |
| SPRR3 | BC017802 | F: ttccacaacctggaaacaca R: ttcagggaccttggtgtagc |
200 |
| SPRR4 | NM 173080 | F: caagtgaagcagccttgtca R: atctggtagccaggatggtg |
222 |
| LEP1 | Marshall et al.5 | F: cctccagtctcttcctgctg R: tgatcactcatttcacccga |
270 |
| LEP6 | Marshall et al.5 | F: ccatctaacttgctgtctgacc R: aggaagatgaagttgcccct |
496 |
| LEP16 | Marshall et al.5 | F: ctttgttttgtccaggacaa R: cctgtggtggttcaggaag |
210 |
| Filaggrin | AF043380 | F: gctctaggcactcagcatcc R: gagccgtctcctgattgttc |
235 |
| Involucrin | NM_005547 | F: ggactgcctgagcaagaatgtg R: taagctgctgctctgggttt |
121 |
| Loricrin | NM000427 | F: gtgggagcgtcaagtactcc R: tagagacgcctccgtagctc |
243 |
| GADPH | M33197 | F: gccaaggtcatccatgacaac R: gtccaccaccctgttgctgta |
498 |