Table 1.
PCR-RFLP conditions for NOS2 gene polymorphisms and gene IL-1β.
| Genes | Primers | Enzyme Time/T° | Fragments | Reference |
|---|---|---|---|---|
|
NOS2 Ser608Leu rs2297518 |
F: 5' - TGTAAACCAACTTCCGTGGTG - 3' R: 5' - GTCTCTGCGGGTCTGAGAAG - 3' |
Tsp509I (10 U/μL) 16 hs/65°C |
288 bp CC:113, 175 bp CT:113, 142 and 175 bp TT:113, 142 bp |
17 |
|
NOS2 -954G/C |
F: 5' - CATATGTATGGGAATACTGTATTTCAG - 3' R: 5' - TCTGAACTAGTCACTTGAGG - 3' |
BsaI (5 U/μL) 1 h/37°C |
573 bp GG:437, 136 bp GC: 573, 437 and 136 bp CC: 573 bp |
18 |
|
NOS2 -1173C/T |
F:5' - CAAAGATCCTTGAGCTCTGA - 3' R:5' - CAACTACATTAGGGAGAAGTTGAG - 3' |
BccI (10 U/μL) 1 h/37°C |
199 bp CC: 199 bp CT: 199, 132 bp TT: 132 bp |
33 |
| IL-1β | F:5' - CATGTGACCTGCTCGTCAGT - 3' R:5' - CCCTAGGGATTGAGTCCACA - 3' |
BccI (10 U/μL) 1 h/37°C |
372 bp 141 and 231 bp |
- |
T°, temperature.