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. 2010 Jun 23;10:30. doi: 10.1186/1472-6882-10-30

Figure 1.

Figure 1

Flowchart of the isolation process for the ethanol extract of bee pollen. Ethanol extract of bee pollen was subjected to silica gel column chromatography eluted with CHCl3 and methanol or Sephadex™ LH-20 column chromatography eluted with methanol. Each column chromatography fractions were further purified by repeated ODS or silica gel column chromatography, finally yielding purified 5 compounds.