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. 2010 Jun 15;8:32. doi: 10.1186/1477-5956-8-32

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Copyright ©2010 Zárate et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Characterization of the time courses of expression and final sizes of the recombinant proteins produced by the hybrid extract. A. Time Course. Cell-free expression reactions were set up within the wells of a microtiter plate, and fluorescence emission was quantified each hour over a four hour period using a Fluoroskan II fluorimeter. B. Protein Product Characterization. After cell-free expression, the target proteins were analyzed by western blotting, using peroxidase-labeled anti-GFP antibody for detection. Expected sizes in kDa: Ankyrin-repeat, 49.8; MBP, 70.2; GFP 27.3; CRY1, 106.4; AXR3, 55.9; SHY2, 52.1; RGA, 95.3; IAA9, 66.2; FYPP3, 62.6; PP2C, 60.5.