Table 1.
Proteins expressed on autofluorescent protein microarrays via hybrid cell-free expression
| DNA/Protein | [Swiss-Prot] | Reference | Locus ID | Clone* | Size (aa) | Fluorescence Intensity (a.u.) |
CV+ |
|---|---|---|---|---|---|---|---|
| Ankyrin-repeat | Q9FF09 | [59] | At5g07840 | U14849 | 175 | 57167 | 13.1 |
| MBP | P0AEX9 | [31] | 368 | 53806.6875 | 14.5 | ||
| GFP | P42212 | [26] | 242 | 48381.375 | 11.9 | ||
| IMB1 | Q56W05 | [60] | At2g34900 | pda11561 | 386 | 37860.1875 | 14.8 |
| IAA9 | Q38827 | [61] | At5g65670 | C00016(E) | 338 | 30984.0625 | 7.8 |
| AXR3 | P93830 | [62] | At1g04250 | pda03374 | 229 | 29581.875 | 8.2 |
| SHY2 | Q38822 | [63] | At1g04240 | C00011(F) | 189 | 28544.3125 | 9.7 |
| GST | P26624 | [32] | 224 | 26364.8125 | 12.9 | ||
| PIF3 | O80536 | [64] | At1g09530 | 524 | 21759.5625 | 17.3 | |
| PIL5 | Q8GZM7 | [65] | At2g20180 | 407 | 19228.25 | 19.3 | |
| ELF3 | O82804 | [66] | At2g25930 | U24077 | 339 | 16343.8125 | 9.8 |
| PP2C | Q9LME4 | [59] | At1g22280 | U19121 | 281 | 10684.125 | 12.5 |
| FYPP3 | Q9LHE7 | [67] | At3g19980 | U21104 | 303 | 8129.5 | 9.5 |
| PixE | P74294 | [34] | 380 | 5983.25 | 10.4 | ||
| TUB1 | Q8GZ16 | [68] | At1g75780 | pda10291 | 447 | 4330.3125 | 9.9 |
| PKS1 | Q9SWI1 | [69] | At2g02950 | U13787 | 439 | 3335.0625 | 9.9 |
| LovR | Q9ABE4 | [33] | 152 | 3312.25 | 9.4 | ||
| CRY1 | Q43125 | [70] | At4g08920 | U12079 | 681 | 3084.25 | 8.8 |
| CCA1 | Q8S8N5 | [71] | At2g46830 | C105127 | 526 | 2739.6875 | 4.7 |
| NACA2 | Q94JX9 | [59] | At3g49470 | U15737 | 217 | 2218.5625 | 7.1 |
| NDPK2 | O64903 | [72] | At5g63310 | U19177 | 231 | 2209.75 | 8.7 |
| PIL6 | Q84LH8 | [73] | At3g59060 | U16079 | 300 | 2166.125 | 5.4 |
| GID1L1 | Q9MAA7 | [74] | At3g05120 | U17384 | 345 | 2143.1875 | 6.7 |
| FHY1 | Q8S4Q6 | [75] | At2g37678 | 202 | 2123.8125 | 3.1 | |
| DET2 | Q38944 | [76] | At2g38050 | U09847 | 262 | 1980.8125 | 3.7 |
| EID1 | Q8LEA8 | [77] | At4g02440 | pda12208 | 336 | 1882.9375 | 7 |
| PCL1 | Q9SNB4 | [78] | At3g46640 | U22928 | 323 | 1821 | 5.6 |
| SLY2 | Q9LUB6 | [79] | At5g48170 | S63202 | 157 | 1747.3125 | 7.8 |
| CRY2 | Q96524 | [80] | At1g04400 | U19559 | 612 | 1715.1875 | 14.4 |
| RGL1 | Q9C8Y3 | [81] | At1g66350 | U18422 | 511 | 1320.0625 | 10.5 |
| RGA | Q9SLH3 | [82] | At2g01570 | U13937 | 587 | 1286.75 | 9 |
| No expression | |||||||
| DNA/Protein | |||||||
| GAI | Q9LQT8 | [74] | At1g14920 | U14047 | 533 | 1190.4375 | 2.8 |
| RGL3 | Q9LF53 | [83] | At5g17490 | U60167 | 523 | 1139.1875 | 1.6 |
| TOC1 | Q9LKL2 | [84] | At5g61380 | U21896 | 618 | 1093.5625 | 3.6 |
| SEC | Q9M8Y0 | [85] | At3g04240 | 977 | 1091.0625 | 4.1 | |
| SPY | Q96301 | [86] | At3g11540 | 914 | 1054.75 | 3.4 | |
| No DNA | 1027.9375 | 3.9 |
*Indicates the cDNA clone used to produce T7 promoter-based DNA constructs for protein expression. Clone names that start with 'pda' are from the RIKEN Bioresource Center, Japan; those starting with U or C were purchased from the Arabidopsis Biological Resource Center, Columbus, OH. If no clone is indicated, the cDNA was obtained from an individual researcher.
+CV: coefficient of variation.