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Optimization of different conditions at laboratory scale shake flask (250 ml). (a) Different media compositions used for the production of 3-nitrocatechol from 3-nitrophenol by strain P. putida F1. (b) pH of the medium. (c) Aeration. (d) Temperature. (e) 3-nitrophenol concentration. (f) Inoculums size. (g) Different media compositions used for the production of 3-methylcatechol from toluene by E. coli expression clone (pDTG602). (h) pH of the medium. (i) Temperature. (j) Aeration.
