Table 1.
Comparison of expression levels and N-glycan composition of SEAP in Sf9, High Five, and Ao38 cells
| Cell line | Sf9 | High Five | Ao38 |
|---|---|---|---|
| Expression level (U/ml) | 6 | 11.6 | 23.2 |
| Identified glycans | Man2, Man3, Man3F, Man4, Man5-8 | Man2, Man3, Man4, Man4F, Man5-8 | Man2F, Man3F, Man4F, Man3F1,2 |
| Man2-6, HexNAc-Man3 | |||
| Man3F: 30% | Man3: 25% | Man3F: 53% for N139 | |
| Major componenta | Man4: 40% | Man4F: 15% | Man6: 31% for N266 |
| Man3: 29% for N266 | |||
a Percent composition of the major component of N-glycans were determined by LC/MS/MS for each glycan (N139 or N266) of Ao38-expressed SEAP, and compared with published data from total glycans of SEAP expressed in Sf9 and High Five cells (see reference [10]).