Table 2.

PCR names, primer sequences and amplification conditions used.

PCR primer name	Primer sequence	Amplification conditions (Taq polymerase; BIOTOOLS , Spain)
HDP2 PCR I: HDP2F2 HDP2R2	5'-GCTGTACCAGCACCTAACCRTCC-3' 5'-GCACACCGCAGCCAATTGGCTG-3'	94°C for 30 sec, 55°C for 30 sec, 72°C for 1 min, 72°C for 7 min (35 cycles)
HDP2 PCR II: PTs7S35F1 HDP2R1	5'-CAGTGGCATAGCAG AGGAGGAA-3' 5'-GCGGAAAGTGGATCCGACTTCGATGG-3'	94°C for 30 sec, 56.5°C for 30 sec, 72°C for 2 min, 72°C for 7 min (35 cycles)
HDP2 PCR III: HDP2F2 HDP2R3	5'-GCTGTACCAGCACCTAACCRTCC-3' 5'-ATCCTGCTCCATGTCGCTTCTAGC-3'	94°C for 30 sec, 60°C for 30 sec, 72°C for 2 min, 72°C for 7 min (35 cycles)