Figure 6.

Effect of Tgfβ treatments on mesodermal proliferation in early limb buds. (A) Representative flow cytometry histograms to evaluate proliferation in limb bud explants of stage HH20/21 cultured for 18 hours in control media or in a medium supplemented with 0.033 μg/ml Tgfβ1 after labeling with Edu. (Left): control unlabeled sample; (middle): control sample untreated with Tgfβ; (right) Tgfβ treated sample. The percentage of labeled cells is indicated in the histogram. (B) Bars graph summarizing the rate of cell proliferation in control and treated limb explants.