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. Author manuscript; available in PMC: 2011 Jul 8.
Published in final edited form as: Immunol Lett. 2010 Mar 25;131(2):126–130. doi: 10.1016/j.imlet.2010.03.004

Figure 1. Macrophages devoid of STAT3 display increased capacity to stimulate cognate responses in naïve CD8 T cells.

Figure 1

A & B. CLN4 CD8 T cells were cultured in vitro for 72 hours with PEM from wild type (WT), Stat3-KO, Stat4 KO or Stat6 KO mice, previously loaded with the class-I dominant epitope of HA, IYSTVASSL at 10ng/mL (or left untreated). . Plots represent CD8+/Vβ8+/Thy1.1+ gated populations depicting proliferation, measured by CFSE dilution, versus IFN-γ production. C. Dose-response effects (white bars –no peptide; gray bars - 1ng/mL peptide pulse; black bars - 10ng/mL peptide pulse) in terms of antigen-specific proliferation as measured by CFSE dilution (top figure) and cytokine production as determined by intracellular cytokine staining (middle and bottom figures).