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. 2010 Jun 15;10:181. doi: 10.1186/1471-2148-10-181

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Copyright ©2010 Herzig et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression profiles of bovine CD163 genes. (A) Bovine ex vivo PBMC (lane 1), ConA-activated PBMC (lane 2), mesenteric lymph node (MLN, lane 3), lung (lane 4) and intestinal epithelial lymphocytes (IELs, lane 5) were evaluated for expression of CD163A and CD163c-α by RT-PCR. GAPDH is shown for comparison. These results are representative of reactions (n = 3) performed for at least two animals. (B) Bovine total PBMC, γδ TCR⁺cells, γδ TCR ^-cells, WC1⁺cells, and WC1^-cells were evaluated for CD163A, CD163c-α, and GAPDH expression by RT-PCR. Templates were serially diluted by a factor of three. These results are representative of two reactions.