Table 1.
NcdT436S kinetics and motility
| Motor | ADP Release* koff (s-1) |
ATPase | Velocity (μm/min) |
|||||
|---|---|---|---|---|---|---|---|---|
| No MTs + ATP |
+ MTs | + MTs, ATP | No MTs kcat (s-1) |
+ MTs kcat (s-1) |
Km,MTs (μM) |
Leading end |
Lagging end |
|
| NcdT436S | 0.0304 ± 0.0005 (n = 6) |
0.014 ± 0.003 (n = 4) |
0.066 ± 0.009 (n = 3) |
0.28 ± 0.10 (n = 4) |
2.2 ± 0.4 (n = 4) |
1.3 ± 0.6 (n = 4) |
6.8 ± 0.1 (n = 22) |
7.6 ± 0.2 (n = 22) |
| WT Ncd | 0.00305 ± 0.00002 (n = 6) |
0.018 ± 0.006 (n = 6) |
0.04 ± 0.01 (n = 6) |
0.10 ± 0.04 (n = 4) |
1.0 ± 0.2 (n = 4) |
1.6 ± 0.8 (n = 4) |
10.2 ± 0.7 (n = 32) |
10.9 ± 0.7 (n = 31) |
MTs, microtubules. Mean ± sem.
*No MTs assays in HEM100; + MTs assays in HEM50