Table 1. PCR primers.
Primer name | Primer sequence |
HBV1798FLong | CTGCGCACCAGCACCATGCAACTTTTTC |
HBV1801RLong | CAGACCAATTTATGCCTACAGCCTCCTA |
HBV1847FS | CGATTTAGGTGACACTATAGAAGAGAGGCTTGTTCATGTCCCACTGTTCAA |
HBV2394RS | CAGTAATACGACTCACTATAGGGAGAAGGCTGGCGAGGGAGTTCTT |
HBV2298FS | CGATTTAGGTGACACTATAGAAGAGAGGCTGACCACCAAATGCCCCTAT |
HBV2933RS | CAGTAATACGACTCACTATAGGGAGAAGGCTTCGGGAAAGAATCCCAGAGGAT |
HBV2821FS | CGATTTAGGTGACACTATAGAAGAGAGGCTGGTCACCATATTCTTGGGAAC |
HBV0272RS | CAGTAATACGACTCACTATAGGGAGAAGGCTTGAGAGAAGTCCACCACGAGT |
HBV0179FS | CGATTTAGGTGACACTATAGAAGAGAGGCTCTAGGACCCCTGCTCGTGTT |
HBV0704RS | CAGTAATACGACTCACTATAGGGAGAAGGCTCGAACCACTGAACAAATGGCACT |
HBV0599FS | CGATTTAGGTGACACTATAGAAGAGAGGCTGTATTCCCATCCCATCATCCTG |
HBV1286RS | CAGTAATACGACTCACTATAGGGAGAAGGCTGCTAGGAGTTCCGCAGTATGG |
HBV1175FS | CGATTTAGGTGACACTATAGAAGAGAGGCTGCCAAGTGTTTGCTGA |
HBV1788RS | CAGTAATACGACTCACTATAGGGAGAAGGCTGCCTACAGCCTCCTA |
SP6 | cgatttaggtgacactatagaagagaggct |
T7 | cagtaatacgactcactatagggagaaggct |
*Numbers within primer names represent the primer positions. An F after the primer position stands for sense primers while R stands for anti-sense primers.
**SP6 and T7 are tag sequences attached at the 5′ end of all PCR primers used in this study. SP6 and T7 primers were used to sequence PCR fragments.