Fig. 3.

Multiple slip-outs interfere with repair. Circular hybrids with homoduplex slipped (CTG)50•(CAG)50 S-DNAs with a unique nick, copurified with fully duplexed forms (background) (SI Appendix, Fig. S1). (Upper) Southern blot analysis of S-DNA repair products using MMR-proficient HeLa and MMR-deficient LoVo extracts. Autorad is from a single gel, identical exposure time, with intervening lanes excised for presentation. (Lower) Repair efficiencies were calculated from three to six replicates. Graph shows starting background (hatched bars) and repair (white bars). See also Figs. S6 and S9 in SI Appendix.