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. 2010 Jul 6;107(28):12704–12709. doi: 10.1073/pnas.0910106107

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Fig. 1. — Neural differentiation of pre-evaluated safe MEF-iPS cells in vitro. (A) Neurospheres derived from EB3 ES cells and 38C2 iPS cells. (Scale bar: 200 μm.) (B) Immunocytochemical analysis of neural cell marker proteins in the differentiated SNSs derived from EB3 ES and 38C2 iPS cells. (Scale bar: 100 μm.) (C) Neural differentiation efficiencies of neurospheres derived from EB3 ES and 38C2 iPS cells. (n = 5, n.s.). (D) RT-PCR analysis of undifferentiated cells (Un.), EBs, PNSs, SNSs, differentiated PNSs (PNS diff.), and SNSs (SNS diff.) of the EB3-ES and 38C2 iPS cells.