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. 2010 Jul 6;107(28):12704–12709. doi: 10.1073/pnas.0910106107

Fig. 5.

Fig. 5.

Characterization and transplantation of SNSs derived from safe and unsafe TTF-iPS cells. (A) Neurospheres derived from 1A2 ES cells, 335D1, 256H13, and 256H18 iPS cells. (Scale bar: 200 μm.) (B) The differentiation potential of TTF-iPS-derived SNSs tested in vitro by immunocytochemical analyses of neural cell markers; Tuj1 for neurons, GFAP for astrocytes, and CNPase for oligodendrocytes. (Scale bar: 100 μm.) (C) Time course of functional recovery of the hindlimbs evaluated by BMS. 335D1 iPS-SNS: n = 9 each; 256H13 and 256H18 iPS-SNS: n = 9; 1A2 ES-SNS: n = 9; PBS control: n = 8. *P < 0.05, **P < 0.01. (D–F) H&E sagittal sections of the spinal cord 42 d after injury. (D) 335D1 iPS-SNS, (E) 256H18 iPS-SNS, and (F) 256H13 iPS-SNS grafted mice. There was no evidence of tumorigenesis in the 335D1 iPS-SNS grafted mice (D), whereas teratoma formation was detected within the injured spinal cord in both 256H18 iPS-SNS (E), and 256H13 iPS-SNS (F) grafted mice. (G) Anti-Nanog DAB staining of sagittally sectioned spinal cord of 256H18 and 256H13 iPS-SNS–transplanted animals 35 d after transplantation.