Fig. 1.
Organization of MLV gag-pol gene. The random insertion library size was 4.3 × 105, estimated by colony counting after transformation of electrocompetent E. coli. Based on the 5,214 nucleotide positions in MLV gag-pol, the library likely covered all possible insertion sites. The ZFD insertion sites of viable clones are indicated by open circles and labels. In general, the name of each clone includes the clone number, the viral protein into which the ZFD is inserted, and the identity of the ZFD. MA, matrix; CA, capsid; NC, nucleocapsid; PR, protease; RT, reverse transcriptase; IN, integrase.