. 2010 Jun 28;107(28):12475–12480. doi: 10.1073/pnas.1001402107

Table 1.

Permissive ZFD insertion sites within MLV Gag and Pol proteins

Clones	Sequence near inserted zinc fingers	Region of insertion
16.p12.zfd1	VLSD¹⁸APZF_domainAGPHSDSGGP	p12
16.p12.zfd2	VLSD¹⁸SAZF_domainASSDSGGP	p12
16.p12.zfd3	VLSD¹⁸SAZF_domainASSDSGGP	p12
52.p12.zfd1	TPSL⁷VRPZF_domainAGPHLGAKP	p12
52.p12.zfd2	TPSL⁷VSAZF_domainASLGAKP	p12
52.p12.zfd3	TPSL⁷VSAZF_domainASLGAKP	p12
14.RT.zfd1	EARL⁷¹RPZF_domainAGPHRLGIKP	RT
14.RT.zfd2	EARL⁷¹SAZF_domainASLGIKP	RT
14.RT.zfd3	EARL⁷¹SAZF_domainASLGIKP	RT
375.RT.zfd1	DVSL²⁰VRPZF_domainAGPHLGSTW	RT
375.RT.zfd2	DVSL²⁰VSAZF_domainASLGSTW	RT
375.RT.zfd3	DVSL²⁰VSAZF_domainASLGSTW	RT
15.IN.zfd1	HVKA³⁷⁶VRPZF_domainAGPQAADPG	IN
15.IN.zfd2	HVKA³⁷⁶VSAZF_domainASGADPG	IN
15.IN.zfd3	HVKA³⁷⁶VSAZF_domainASGADPG	IN
17.IN.zfd1	KYWV³⁶RPZF_domainAGPHWVYQGK	IN
17.IN.zfd2	KYWV³⁶SAZF_domainASVYQGK	IN
17.IN.zfd3	KYWV³⁶SAZF_domainASVYQGK	IN
273.IN.zfd1	GAPP²⁷²LRPZF_domainAGPHPPLVN	IN
273.IN.zfd2	GAPP²⁷²LSAZF_domainASPPLVN	IN
273.IN.zfd3	GAPP²⁷²LSAZF_domainASPPLVN	IN

The clone number is followed by the viral protein into which the ZFD was inserted, and the identity of the ZFD. The notations zfd1, zfd2, and zfd3 indicate clones containing a ZFD originally engineered to bind to a 18-bp stretch that appears in the promoter regions of γ-globin, PEDF, and CHK2 genes, respectively (15–17). The positions of amino acid residues flanking the ZFD insertion are also indicated, with the residues of wild-type MLV Gag-Pol proteins, and amino acids additionally introduced via the transposon reaction and cloning steps (underlined).