Fig. 2.

dmPTB protein exhibits sexually dimorphic expression and is predominantly expressed in primary spermatocytes. (A) dmPTB antibodies specifically recognize dmPTB protein (recombinant and endogenous). (B) (Top) Western blot analysis reveals that the two major isoforms of dmPTB are predominantly expressed in wild-type males but not females or heph² males. (Middle) Overexposure reveals low-level expression of dmPTB isoforms in wild-type females and heph² males. (Bottom) Anti-actin is loading control. (C) Antibody staining reveals that dmPTB is predominantly expressed in the nuclei of primary spermatocytes of wild-type males. Low-level expression of dmPTB is observed in the nuclei (at the bulged tip testis) of primary spermatocytes in heph² males.