Fig. 4.

dmPTB is necessary for proper functioning of the spermatid IC. (A) Loss of dmPTB in the heph² mutant results in numerous bulges (arrows) along the length of partially individualized spermatids, a phenotype characteristic of individualization defects. (B) Loss of dmPTB results in interruption of the actin cones of the IC. Anti-phalloidin staining in heph² males shows that the register of the actin cones is disrupted compared with wild-type actin cones. Anti-DAPI staining indicates that the nuclei of individualizing spermatids in heph² mutants are disrupted and scattered along the cyst.