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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: FEMS Microbiol Lett. 2010 May 14;309(1):55–61. doi: 10.1111/j.1574-6968.2010.02013.x

Fig. 1.

Fig. 1

Interactions of recombinant Scl1 constructs with human plasma HDL. Human plasma was applied to affinity columns containing immobilized rScl1 constructs C176, C176V, and C176T or to a control column without rScl protein. Protein complexes eluted from the columns were precipitated with TCA. (a) Schematic representation of rScl1 constructs (not to scale). V, variable region; CL, collagen-like region; L, linker region. (b) SDS-PAGE analysis. Eluted fractions from columns were analyzed by 15% SDS-PAGE stained with RAPIDstain. HDL preparations were used as marker. rScl constructs were marked with solid triangles. Common ~25-kDa band is marked with open triangles. (c) Western blot analysis. Proteins shown in (a) were transfered onto a nitrocellulose membrane. Presence of ApoAI was tested with anti-ApoAI antibody (upper panel) and rScl1 were detected with a Strep-Tactin-HRP conjugate (bottom panel). Immunoreactivity was visualized using chemiluminescence substrate.