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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: FEMS Microbiol Lett. 2010 May 14;309(1):55–61. doi: 10.1111/j.1574-6968.2010.02013.x

Fig. 2.

Fig. 2

Interactions of recombinant Scl1 constructs with purified HDL. Commercial HDL was applied to affinity columns containing immobilized rScl1 constructs C176, C176V, and C176T or to control column without rScl1 protein. Protein complexes eluted from the columns were TCA-precipitated. Protein samples eluted from columns were analyzed by 15% SDS-PAGE stained with RAPIDstain (upper panel). HDL preparations were used as marker. The rScl1s were marked with solid triangles. Common ~25-kDa band was identified as ApoAI (open triangles). Proteins shown in upper panel were transferred onto a nitrocellulose membrane. Presence of ApoAI was tested with anti-ApoAI antibody. Immunoreactivity was visualized using chemiluminescence substrate (bottom panel).