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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: FEMS Microbiol Lett. 2010 May 14;309(1):55–61. doi: 10.1111/j.1574-6968.2010.02013.x

Table 1.

Strains, plasmids, recombinant proteins and primers used in the present study.

Strains, plasmids or recombinant protein Source or application
Strains Souce
 ATCC12373 ATCC
 E. coli BL21 Shanghai Sangon
Plasmids
 pASK-IBA2 IBA-GmbH
Recombinant proteins
 C176 This work
 C176V This work
 C176T This work
Primers Amplification
 C176F: 5′-atggtaggtctcaggccgatatctgggaccaggagcaaag-3′ C176 and C176V
 C176R: 5′-atggtaggtctcagcgctttgttcgcctggttgctctggc-3′ C176 and C176T
 C176VR: 5′-atggtaggtctcagcgctacgtaaagcatcgctcttaagacc-3′ C176V
 C176TF: 5′-atggtaggtctcaggccagttatgatagcgtagaactttataat-3′ C176T