Fig 1.
Genetic mapping of the K locus. (A) Initial linkage studies [phase 1, (14)] defined a 12-Mb critical region for KB; ascertainment and characterization of additional kindreds narrowed the interval to 3.8 Mb (phase 2, figs. S1 to S4). Association analysis for 60 markers in brindle (n = 12) versus yellow (n = 10) Boxers, and for 51 markers in black (n = 9) versus yellow (n = 10) Great Danes, was carried out as described in the text. (B) Candidate genes in the 320-kb region of greatest association in Great Danes; this region includes 12 β-defensin genes (shown in red). (C and D) Significance, plotted as −log of P values from a chi-square test of allele counts, is shown as a function of distance along CFA16 (only for SNPs present at greater than 10% frequency and genotyped in at least 75% of the samples). The dashed red line indicates a Bonferroni-corrected 5% significance level; these regions are indicated by hatched and black bars for Boxers and Great Danes, respectively, in (A). Annotation is based on the Non-dog RefSeq track in the UCSC Genome Browser, except for CBD102, identified by Patil et al. (16).