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. Author manuscript; available in PMC: 2010 Jul 19.
Published in final edited form as: Science. 2007 Oct 18;318(5855):1418–1423. doi: 10.1126/science.1147880

Fig 3.

Fig 3

Expression of β-defensin mRNA and protein in skin and in cultured keratinocytes. (A) Levels of Agouti, CBD1, or CBD103 mRNA from black or yellow dog skin, as indicated, determined relative to Bactin by quantitative RT-PCR, and expressed as percentage of mRNA present in the yellow samples. Results shown represent the mean ± SEM of four different animals. (B) Expression of epitope (V5)–tagged CBD103 (+) or epitope (V5)–tagged CBD103ΔG23 (ΔG23) in cell layer and media after transfection of mouse keratinocytes as determined by Western blotting with antisera against the V5 epitope. Representative results are shown for one of four experiments; for each experiment, the two constructs were transfected in triplicate or quadruplicate.