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. Author manuscript; available in PMC: 2011 Jul 16.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Jun 15;398(1):56–61. doi: 10.1016/j.bbrc.2010.06.032

Fig. 2.

Fig. 2

DFO alters endogenous HIF-1α and hMOR message levels

RNA was extracted from cells treated without (control, C) or with DFO (A-C) for 24 hrs, or with DFO for 0, 2, 4, 8, 12 or 24 hrs (D-E). RT-PCR was performed using human HIF-1α (A, D), MOR (B, E) or DOR (C)-specific primers. Human β-actin specific primers were included in every PCR reaction (added at the cycle 19) as an internal control for normalization purpose. The normalized message from control, or from cells at time zero, was arbitrarily defined as 100%. Quantitative analysis of message levels are presented as mean ± S.E. “*” indicates p< 0.01. (student paired t-test).