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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Cancer Gene Ther. 2010 May 21;17(8):579–584. doi: 10.1038/cgt.2010.14

Fig.3.

Fig.3

Autophagy is produced as measured with LysoTracker Red DND-99 and flow cytometry. Ad-IFNα infected cells were labeled with DND and analyzed on channel APC-A. Representative histograms are displayed. Data overlay was done by FlowJO. On the y axis is the % Max (the cell count in each bin divided by the cell count in the bin that contained the largest number of cells), and the x axis is the fluorescence intensity in log scale. Both normal and KU7 cancer cells show a shift to the right after Ad-IFNα treatment as a measure of autophagy (A and B, respectively) whereas none is seen in the KU7 cells exposed to conditioned medium obtained 72hr after KU7 cell exposure to Ad-IFNα (KU7 CM-Ad-IFNα) compared to cells treated with KU7 conditioned medium from untreated cells at the same time point (KU7 CM-control).