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. Author manuscript; available in PMC: 2011 Jul 12.
Published in final edited form as: Vaccine. 2010 May 21;28(31):5017–5022. doi: 10.1016/j.vaccine.2010.05.018

Fig. 2.

Fig. 2

Human serum and plasma block the binding of J14 antibodies but not hexavalent antibodies to the surface of GAS. After blocking non-specific sites on the surface of GAS with PBS-tween plus 5% BSA, normal human serum (▤), human plasma (▧) or PBS (□) were then incubated with the bacteria. Hexavalent (serum #2, table 1) and J14 (titer=25,600 against J14) rabbit sera (1:200) were then added to the adherent bacteria, incubated, washed and followed with HRP-goat anti-rabbit IgG. After washing, ABTS (KPL, Gaithersburg, MD) was used to quantify detectable rabbit hexavalent and J14 antibodies.