Table 1.
Rabbit #2 | ELISA titer against1: |
||||||||
---|---|---|---|---|---|---|---|---|---|
Hexa | Hexa-J14 | J14 | M24.2 | M5.2 | M6.6 | M19.2 | M1-0.2 | M3.2 | |
Pre-pool | <200 | <200 | <200 | <200 | <200 | <200 | <200 | <200 | <200 |
#1 | 51,200 | 51,200 | <200 | 6,400 | 6,400 | 25,600 | 6,400 | 3,200 | 1,600 |
#2 | 102,400 | 102,400 | <200 | 25,600 | 12,800 | 51,200 | 6,400 | 12,800 | 6,400 |
#3 | 204,800 | 204,800 | <200 | 25,600 | 25,600 | 51,200 | 6,400 | 12,800 | 12,800 |
Pre-pool | <200 | <200 | <200 | <200 | <200 | <200 | <200 | <200 | <200 |
#4 | 51,200 | 102,400 | 3,200 | 12,800 | 12,800 | 51,200 | 6,400 | 6,400 | 3,200 |
#5 | 51,200 | 51,200 | 1,600 | 6,400 | 6,400 | 25,600 | 3,200 | 3,200 | 6,400 |
#6 | 204,800 | 204,800 | 1,600 | 25,600 | 25,600 | 51,200 | 6,400 | 6,400 | 6,400 |
ELISA antigens were the purified recombinant vaccine proteins (hexavalent and hexavalent-J14), the unconjugated synthetic J14 peptide or recombinant dimeric peptides that copied the type-specific components of the hexavalent hybrid protein.
Rabbits #1–#3 were immunized with 200μg recombinant hexavalent protein and rabbits #4–#6 received i.m. injections of 200μg of recombinant hexavalent-J14 protein at times 0, 4 and 8 weeks. Serum was obtained 2 weeks after the final immunization.