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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2010 Mar 19;121(1-2):159–163. doi: 10.1016/j.jsbmb.2010.03.055

Figure 4.

Figure 4

Effect of 1,25(OH)2D3 on interaction between VDR and Caveolin-3. Isolated cardiomyocytes from adult rat heart were treated in culture with 1 nM 1,25(OH)2D3 or ethanol (control) for 1 hour before protein extraction. 600 ug of protein lysates each were incubated with VDR monoclonal (MA1-710) and polyclonal (sc-1008) antibody-coupled gel resins, caveolin-3 polyclonal (PA1-066) antibody-coupled gel resin, and control gel resin respectively. Eluted fractions of IPed protein samples (20 ul each) were subjected to Western blot analysis. A, Western blot of VDR antibody IPed samples. The blot was first probed with Caveolin-3 antibody (sc-5310), then striped and reprobed with VDR antibody (sc-1008). B, Western blot of caveolin-3 antibody IPed samples. The blot was first probed with VDR antibody (sc-1008), then striped and reprobed with caveolin-3 antibody (sc-5310).