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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Cancer Gene Ther. 2010 May 7;17(8):559–570. doi: 10.1038/cgt.2010.11

Figure 2.

Figure 2

Specific binding of expressed anti-HER2 antibody to HER2 positive cells. Medium were collected from 293 cells transfected with pAAVαHER2, pAAVαHER2-myc and pAAVαPA-myc, respectively and added to fixed HER2 positive SK-BR-3 cells or fixed HER2 negative BEAS-2b cells. HER2 specific binding was assessed by immunofluorescent microscopy with Alexa 488 labeled goat anti-mouse second antibody. A. SK-BR-3 cells incubated with αHER2; B. SK-BR-3 cells, αHER2-myc; C. SK-BR-3 cells, αPA-myc; D. BEAS-2b, αHER2; E. BEAS-2b cells, αHER2-myc; and F. BEAS-2b cells, αPA-myc. All panels, bar = 20 μm.