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. 2010 Mar;2(3):98–110. doi: 10.1002/emmm.201000062

Figure 2. Stat5- but not Stat3-deletion blocks cell proliferation and induces apoptosis in vitro.

Figure 2

  • A, B. PCR analysis of gene deletion after IFN-β treatment. Deletion efficiency was determined by a PCR for floxed (fl) and deleted (Δ) Stat3 (A) and Stat5 (B) alleles 48 h after IFN-β treatment.
  • C, D. Effect of Stat5 or Stat3 deletion on cell proliferation in vitro. (C) Stat3fl/flMx1Cre cell lines were either treated with 1000U IFN-β or mock-treated (n = 3 each). Stat3fl/fl cell lines were used as a control (n = 3). (D) Stat5fl/flMx1Cre cell lines (n = 3 each) were treated analogously to (C).
  • E–H. Cell cycle and apoptosis analysis of IFN-β treated v-abl-transformed cell lines, 2 and 9 days after IFN-β treatment, respectively. Deletion of Stat5 induces a cell cycle arrest (F) and apoptosis (H), while deletion of Stat3 has no significant effect on evaluated parameters (E, G). Numbers show percentages of cells in indicated cell cycle phases (E, F) or in different stages of apoptosis (G, H). Re-expression of Stat5 target genes (D-type cyclins, c-myc, bcl-xL or bcl-2) failed to rescue Stat5-deficiency (I).