Figure 5.
Immunoblot analysis of β-dystroglycan and associated components in normal and dystrophic skeletal muscle fibres. Shown are identical immunoblots labeled with antibodies to laminin (LAM) (a), α-dystroglycan (α-DG) (b), β-dystroglycan (β- DG) (c), α-sarcoglycan (α-SG) (d), full-length dystrophin of apparent 427 kDa (Dp427) (e), and the α1-subunit of the dihydropyridine receptor (α1-DHPR) (f). Lanes 1 to 3 represent microsomal membranes isolated from normal muscle fibres, mdx fibres, and mdx-3cv fibres, respectively. The position of immuno-decorated protein bands is indicated by arrow heads.