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. 2010 Jun 15;103(2):265–274. doi: 10.1038/sj.bjc.6605711

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Copyright © 2010 Cancer Research UK

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Methylation in the DUSP16 CpG island in BL cell lines and primary BL, but not in LCLs. (A) Sample bisulphite sequencing trace, showing complete methylation in Rael, partial methylation in DG75 and absence of methylation in the LCL. The arrows denote individual CpG dinucleotides numbered from the start of the CpG island. (B) Summary of bisulphite sequencing analysis of DUSP16 CpG island in BL and LCL. The figure shows a diagrammatic representation of the DUSP16 CpG island. CpG sites are shown as vertical lines. Methylated CpG dinucleotides are shown as black blocks, unmethylated CpGs as open blocks. Five levels of methylation are indicated: 0=no black blocks; 1–25%=one black block; 25–50%=two black blocks; 50–75%=three black blocks; 75–100%=four black blocks. Positions of the MSP and bisulphite sequencing primers are indicated. The CpG island is almost completely methylated in Rael, Dante and Ramos, partially methylated in DG75 and Mutu and unmethylated in the LCLs, consistent with expression of DUSP16 mRNA in each cell line. (C) The DUSP16 CpG island is methylated in primary endemic BL. MSP was performed as described in Materials and Methods section. The figure shows unmethylated (U) and methylated (M) MSP reactions for each DNA sample, with control unmethylated (C_U) and methylated (C_M) reactions as shown.