Figure 1.

NLCQ-1 is an effective hypoxic cytotoxin against KHT cells in vitro, but does not impair KHT tumour growth in vivo. KHT cells (A) were exposed to NLCQ-1 under aerobic or anoxic conditions for 3 h. Proliferation was assessed 4 days later by MTT assay. Data points represent the average relative proliferation compared with untreated control cells from three experiments (±s.d.). Exposure to anoxia alone did not influence KHT proliferation (average OD₅₄₀ aerobic controls 1.4 vs 1.3 for anoxic controls). (B) KHT cells were inoculated into C3H mice. Once tumours had established NLCQ-1 was administered daily for 4 consecutive days (15 mg kg^–1). Presented are average tumour volume measurements (±s.e.; n=8 per group).