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. Author manuscript; available in PMC: 2011 Jul 16.
Published in final edited form as: Brain Res. 2010 May 6;1344:1–12. doi: 10.1016/j.brainres.2010.04.069

Figure 7. CREB and P-CREB from the rat pituitary gland bound the rat CART CRE cis-regulatory element in EMSA/SS assays.

Figure 7

Nuclear proteins were isolated from rat pituitaries, and binding to the CART CRE DNA cis-regulatory site was observed by electrophoretic mobility shift assays (EMSA) and antibody super shifts (SS) using an oligonucleotide identical in sequence to the rat CART promoter CRE site, and CREB and P-CREB antibodies. See figure and text for additional details.