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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Bioorg Med Chem. 2009 Nov 27;18(2):809–821. doi: 10.1016/j.bmc.2009.11.046

Figure 6.

Figure 6

LC–MS/MS analysis of free estrogen in cell culture supernatant after four days incubation of E2–BSA or 7EqCD. Positive ion electrospray selected reaction monitoring (SRM) chromatograms of estrogens as dansyl derivatives: (A) EE2 (70 nM) incubated in Ishikawa cell culture (peak intensity 3.0 × 105); (B) 7EqCD (10 nM) incubated with Ishikawa cells (maximum y-axis intensity 1.4 × 103) – EE2 peak was not observed above detection limits (<50 pM); SRM conditions EE2–dansyl·[MH+] m/z 530→171 collision energy 53 eV; (C) E2 (70 nM) incubated in Ishikawa cell culture (peak intensity 5.5 × 105); (D) E2–BSA (1 μg/mL) incubated with Ishikawa cells (peak intensity 1.1 × 104) showing approximately 1 nM based upon standard curves; SRM conditions E2–dansyl [MH+] m/z 506→171 collision energy 57 eV.