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Effect of the A286W mutation on the sensitivity to NSC56452 inhibition. Mixed CHO cell culture expressing either wild type or A286W-EGF receptors were pre-incubated with 1%DMSO (control) or NSC56452 at the indicated doses for 20 min at room temperature before stimulation with 3 nM EGF for 1 minute. Data shown are representative of two separate experiments.
