Table 1.
Activity analysis of IP5 2-K mutants
| IP5 2-K mutant | Activity (% wild type) | |
| Native | 100.00 | |
| R40V* | 56.06 | |
| E85A* | 124.24 | |
| R130I | 6.06 | (9.09) |
| K168A | nd | (2.27) |
| K168N | nd | (4.54) |
| K170S | 5.30 | (3.78) |
| N238A* | 85.60 | |
| D368A | nd | (nd) |
| D407A | 3.03 | (7.57) |
| K411A | nd | (3.53) |
ND: No activity detected. Data in parentheses shows values obtained at 10-fold higher concentration of native.
*Native ATP Km = 38 μM and Vmax = 1080 pmol/ min /μg. The Km value increases 2- to 3-fold for the active mutants (R40V, E85A, and N238A), with < 20% reduction in Vmax.