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. 2010 May 10;107(21):9747–9752. doi: 10.1073/pnas.0911175107

Fig. 2.

Fig. 2.

Mitochondrial dysfunction in DKO mutant flies. (A) The relative level of mtDNA in young (3 days) and old (30 days) control (white) and DKO (gray) thoraces (mean ± SEM, three DNA extractions for 3 days, four for 30 days). The ND5 locus was used to measure mtDNA levels, normalized to the nuclear let-7 locus. P = 0.0055 (t test, two tailed). (B) Western immunoblot for level of complex I subunit NDUFS3 (Upper) and actin shows similar levels in control and DKO with age. (C) Mitochondrial RCR of control (white) and DKO (gray). (Left) Whole flies (mean ± SEM, three preparations of mitochondria for 3 days, four for 30 days) (two-tailed t test, P = 0.005 between 30-day control and DKO samples). (Right) Heads (mean ± SEM, seven preparations of mitochondria). Mitochondrial RCR showed significance in aged whole fly and a modest but insignificant decrease in aged heads RCR (two-tailed t test, P = 0.096 between 30-day control and DKO). (D) ATP levels (mole/mg) of control (white) and DKO (gray) flies (mean ± SEM, three sample preparations) (two-tailed t test, P = 0.011 between 30-day control and DKO samples from whole flies).