Fig. 4.
The RNA-binding domain in RNPC1 is required for binding p63 transcript and for inhibiting p63 expression. (A) Schematic illustration of ΔRNP1 and ΔRNP2 mutants. (B) The RNA-binding domain in RNPC1a is required for binding p63 3′UTR. REMSA assay was performed as in Fig. 3A by incubating 32P-labeled probe C with GST, GST-HA-RNPC1, GST-HA-ΔRNP1, or GST-HA-ΔRNP2. (C) RNP1- and RNP2-deletion mutants are unable to inhibit p63 expression. The level of ΔNp63α along with actin was measured in HaCaT cells transfected with an empty vector or a vector expressing HA-tagged RNPC1a, RNPC1b, ΔRNP1, or ΔRNP2. The relative level of p63 proteins was shown below the lane.