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. 2010 May 10;107(21):9903–9908. doi: 10.1073/pnas.1004515107

Fig. 1.

Fig. 1.

Single-Cys LacY mutants. Positions of Cys replacements are shown on the backbone of LacY (Protein Data Bank ID code 1PV7; www.pdb.org) viewed perpendicular to the membrane with the N-terminal helix bundle on the left and the C-terminal bundle on the right. Black spheres indicate single-Cys replacements on the cytoplasmic side of the sugar-binding site: 60, 67 (helix II), 279 (helix VIII), 329, and 331 (helix X); grey spheres indicate single-Cys replacements on the periplasmic side of the sugar-binding site: 31 (helix I), 42, 44, (helix II), 242, and 245 (helix VII). The light grey spheres at the apex of the inward-facing cavity represent TDG.