Fig. 4.

Structure of pol II transcribing complex stalled at a site-specific pyriplatin-DNA adduct after CMP incorporation. (A) The newly incorporated matched CMP is highlighted in yellow. Other colors are as in Fig. 2. Interactions of the damaged nucleotide and pol II residues are highlighted in (B). The view is taken roughly from an ∼90 degree clockwise rotation along the RNA/DNA helix axis from A. Nitrogen and oxygen atoms are depicted in blue and red, respectively. Hydrogen bonds between ammine group of the cis-{Pt(NH₃)₂(py)}²⁺ moiety and bridge helix residues are shown as black dashed lines. The loop of Rpb2 828–834 is shown in green.