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vFGF affects tracheal BL integrity. Infected larvae were dissected at the indicated hours postinfection. (A) Tracheal laminin (red) was detected using anti-laminin. (B–E) Lysates from infected midguts were immunoblotted using antibodies that recognize laminin (B), type IV collagen (C), eGFP (D), or GAPDH (E). (F) Mock-infected or virus-infected tissues were observed using TEM. BL (arrow), tracheal epithelial cells (t), and trachea lumen (l) are indicated.
