Fig. 2.

Engineering of K238 mutant strains that grow photosynthetically. (A) Selection for photosynthetic growth of mutant strains. After transformation, cells were grown on BG11 media supplemented with antibiotics (kanamycin and chloramphenicol). Mutant strains are indicated below each well. (B) Immunoblot of isolated thylakoid membranes (1 μg chlorophyll/lane). Strains are indicated on the top of each lane. The antibodies probed for targets representing the major photosynthetic complexes in the thylakoid membrane (indicated on left). The TD34 strain, lacking all three psbA genes and therefore the PSII, is also deficient in the amount of PSI.