Table 1.
Fluorescence lifetimes and distances
| Protein | Ligand/state | Donor lifetime (μs) | Sensitized emission lifetime (μs) | Distance (Å) |
| ΔN*-GluR4-G740C | Apo/resting | 1526 ± 50 | 188 ± 12 | 25 ± 0.3 |
| 535 ± 15 | 32 ± 0.3 | |||
| ΔN*-GluR4-G740C | Glu/desensitized | 1510 ± 45 | 173 ± 10 | 25 ± 0.3 |
| 534 ± 18 | 32 ± 0.3 | |||
| ΔN*-GluR4-G740C | Glu+CTZ/open | 1534 ± 45 | 73 ± 10 | 21 ± 0.5 |
| 439 ± 15 | 30 ± 0.3 | |||
| ΔN*-GluR4-G740C-L484Y | Apo | 1841 ± 50 | 63 ± 5 | 20 ± 0.3 |
| 336 ± 18 | 27 ± 0.3 | |||
| ΔN*-GluR4-G740C-L484Y | Glu | 1845 ± 60 | 67 ± 3 | 20 ± 0.2 |
| 357 ± 20 | 28 ± 0.3 | |||
| ΔN*-GluR4-G740C-L484Y | Glu+CTZ/open | 1845 ± 60 | 61 ± 8 | 20 ± 0.5 |
| 444 ± 21 | 29 ± 0.3 | |||
| ΔN*-GluR4-S741C | Apo/resting | 1682 ± 50 | 132 ± 9 | 23 ± 0.3 |
| 595 ± 23 | 32 ± 0.3 | |||
| ΔN*-GluR4-S741C | Glu/desensitized | 1686 ± 60 | 126 ± 10 | 23 ± 0.3 |
| 522 ± 20 | 31 ± 0.3 | |||
| ΔN*-GluR4-S741C | Glu+CTZ/open | 1612 ± 60 | 60 ± 7 | 20 ± 0.4 |
| 367 ± 15 | 29 ± 0.3 | |||
| ΔN*-GluR4-S741C-L484Y | Apo | 1877 ± 45 | 53 ± 9 | 19 ± 0.6 |
| 542 ± 19 | 30 ± 0.3 | |||
| ΔN*-GluR4-S741C-L484Y | Glu | 1820 ± 45 | 55 ± 10 | 20 ± 0.6 |
| 565 ± 17 | 31 ± 0.3 | |||
| ΔN*-GluR4-S741C-L484Y | Glu+CTZ/open | 1818 ± 50 | 51 ± 10 | 19 ± 0.6 |
| 584 ± 15 | 31 ± 0.3 | |||
| ΔN*-GluR4-Th-S741C-Th | Apo/resting | 1578 ± 45 | 188 ± 13 | 25 ± 0.3 |
| 535 ± 16 | 31 ± 0.3 | |||
| ΔN*-GluR4-Th-S741C-Th | Glu/desensitized | 1505 ± 45 | 174 ± 4 | 25 ± 0.2 |
| 534 ± 20 | 32 ± 0.3 | |||
| ΔN*-GluR4-Th-S741C-Th | Glu+CTZ/open | 1579 ± 55 | 73 ± 3 | 21 ± 0.2 |
| 439 ± 15 | 30 ± 0.3 | |||
| ΔN*-GluR4-Th-S741C-Th | ZK200775 | 1679 ± 45 | 77 ± 5 | 21 ± 0.2 |
| 489 ± 15 | 30 ± 0.3 | |||
| ΔN*-GluR4-Th-S741C-Th-L484Y | Apo | 1609 ± 60 | 70 ± 1 | 21 ± 0.1 |
| 588 ± 7 | 32 ± 0.3 | |||
| ΔN*-GluR4-Th-S741C-Th-L484Y | Glu | 1565 ± 55 | 70 ± 1 | 21 ± 0.1 |
| 582 ± 9 | 32 ± 0.3 | |||
| ΔN*-GluR4-Th-S741C-Th-L484Y | Glu+CTZ/open | 1579 ± 50 | 70 ± 1 | 21 ± 0.1 |
| 558 ± 8 | 32 ± 0.3 | |||
| ΔN*-GluR4-S742C | Apo/resting | 1704 ± 60 | 124 ± 7 | 23 ± 0.3 |
| 596 ± 22 | 32 ± 0.4 | |||
| ΔN*-GluR4-S742C | Glu/desensitized | 1730 ± 60 | 118 ± 6 | 23 ± 0.2 |
| 517 ± 15 | 30 ± 0.3 | |||
| ΔN*-GluR4-S742C | Glu+CTZ/open | 1682 ± 55 | 62 ± 5 | 20 ± 0.3 |
| 444 ± 15 | 29 ± 0.3 | |||
| ΔN*-GluR4-S742C-L484Y | Apo | 1557 ± 45 | 66 ± 5 | 21 ± 0.3 |
| 464 ± 15 | 30 ± 0.3 | |||
| ΔN*-GluR4-S742C-L484Y | Glu | 1517 ± 45 | 67 ± 5 | 21 ± 0.3 |
| 477 ± 15 | 31 ± 0.3 | |||
| ΔN*-GluR4-S742C-L484Y | Glu+CTZ/open | 1509 ± 45 | 64 ± 5 | 21 ± 0.3 |
| 446 ± 15 | 30 ± 0.3 |
CTZ, cyclothiazide; Glu, glutamate.