Table 1.
Interaction of Hfq, sRNAs, and rpoS mRNA
| kobs rpoS•sRNA, min-1 | ||||||||
| KsRNA•Hfq, μM | Kd rpoS•sRNA, nM | WT rpoS | (AAN)4 mutant | |||||
| sRNA | KH1 | KH2 | −Hfq | +Hfq | −Hfq | +Hfq | −Hfq | +Hfq |
| DsrA | 0.16 | 0.43 | 11 | 7.5 | 0.18 | 0.68 | 0.09 | 0.24 |
| RprA | 0.11 | 0.42 | 190 | 3.3 | 0.12 | 0.29 | 0.12 | 0.14 |
| ArcZ121 | 0.09 | 0.45 | — | — | — | — | — | — |
| ArcZ56 | — | — | 450 | 0.39 | 0.08 | 2.8 | 0.09 | 1.2 |
sRNA•Hfq and sRNA•rpoS binding assayed by native PAGE. Equilibrium and rate constants were obtained as described in Materials and Methods. See Figs. 3 and 4, and Figs. S6 and S7 for equations and data on which these numbers are based.