Fig. 4.
Channel NH3 transport in proteoliposomes. (A) Relative vesicular pH change indicated by CF fluorescence for RhCG at protein to lipid ratio 1∶90 by weight (open blue triangles) and 1∶45 (closed blue triangles) demonstrated protein dependent NH3 permeability relative to empty liposomes (black squares) and a control protein YaaH (red diamonds). (B) Proteoliposomes from the same sample preparations after addition of 275 mM sucrose at t = 0 were monitored for transport of water resulting in decreased vesicular volume, increased CF concentration, and subsequent self-quenching. Data displayed for the exponential fluorescence decay after lag time to reach self-quenching concentration and exponential fit (black lines).