Fig. 1.

MIG12 overexpression in mice increases FA synthesis resulting in fatty liver. Five mice were injected with adenoviruses (10¹¹ particles/mouse) that expressed β-gal or MIG12. Each value represents the average ± S.E. of values from 5 mice and P values were determined using Student’s t-test. (A) In vivo rates of FA and cholesterol synthesis in livers of mice injected with β-gal or MIG12 adenovirus. Five days after adenoviral injections, mice were injected intraperitoneally with [³H]H₂O (50 mCi) and ³H incorporation into newly synthesized FAs and cholesterol were measured as described in Methods. Similar results were obtained in 1 independent study. (B) Liver TG and cholesterol concentrations were measured 5 days after adenoviral injections. Similar results were obtained in three independent studies.